Investigating Whether HTZ-1 Is Located at the ADE2-TEL Gene in S. Cerevisiae Strain UCC1371

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In S. cerevisiae, the HTZ-1 variant commonly substitutes the H2A histone in heterochromatin regions. HTZ-1 is known to bind to the basal promoter region (TATA box) of the gene. Our study is looking at the possible association of HTZ-1 with an artificially placed telomeric gene, ADE2, within the S. cerevisiae mutant UCC1371. We were able to gain insight on this plausible interaction between the two by utilizing chromatin immunoprecipitation (ChIP) and qPCR technology. From our qPCR results we can speculate that HTZ-1 may be located at the ADE2 gene.

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Title: Investigating Whether HTZ-1 Is Located at the ADE2-TEL Gene in S. Cerevisiae Strain UCC1371
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Subject: Saccharomyces cerevisiae--Transcription factors
Description: In S. cerevisiae, the HTZ-1 variant commonly substitutes the H2A histone in heterochromatin regions. HTZ-1 is known to bind to the basal promoter region (TATA box) of the gene. Our study is looking at the possible association of HTZ-1 with an artificially placed telomeric gene, ADE2, within the S. cerevisiae mutant UCC1371. We were able to gain insight on this plausible interaction between the two by utilizing chromatin immunoprecipitation (ChIP) and qPCR technology. From our qPCR results we can speculate that HTZ-1 may be located at the ADE2 gene.
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Date: 2011
Type: Academic Paper
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Identifier: fortlewis:12190
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Language: English
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Rights: http://rightsstatements.org/page/CNE/1.0/?language=enlocalperson:10874Michael K. Ziemkeperson:10709Spencer DeBaucheThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). If you are a rights holder and believe that our inclusion of certain material in this digital repository violates your rights, please contact: Library Director, John F. Reed Library, 1000 Rim Drive, Durango, CO 81301, library@fortlewis.edu, (970) 247-7315. When you notify us of an alleged copyright infringement, please be sure to include the following information: Your contact information, including email address, telephone and/or mailing address; A detailed description of the infringing material, including the exact URL where you found it; A statement that you in good faith believe that use of the material in the manner complained of is not authorized by the copyright owner, its agent, or the law; A statement by you that the information in the notification is accurate and that you have the authority to enforce the copyrights that are claimed to be infringed.Englishorganization:25Fort Lewis College

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Investigating Whether HTZ-1 Is Located at the ADE2-TEL Gene in S. Cerevisiae Strain UCC1371

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